COPS2 antibody (C-Term)

Catalog No. ABIN374326

Product Details anti-COPS2 Antibody

Target: COPS2 (COP9 Constitutive Photomorphogenic Homolog Subunit 2 (Arabidopsis) (COPS2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This COPS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-LNSLNQAVVS KLA

Specificity: This antibody detects COPS2/TRIP15 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog) and Cow.
Species reactivity (tested):Human and Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from C Terminus of the protein sequence according to NP_004227.1, NP_001137359.1. Genename: COPS2

Application Details

Application Notes: Peptide ELISA: Antibody detection limit dilution 1/16000. Western blot: 1-3 μg/mL. Approx.48 kDa band observed in Human Brain, Mouse Brainlysates and in lysates of Mouse putuitary cell line NIH3T3 (calculated MW of 51.8 kDaaccording to NP_004227.1). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Thyroid Gland shows nclear and cytoplasm staining inactivated epithelial cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for COPS2

Target: COPS2 (COP9 Constitutive Photomorphogenic Homolog Subunit 2 (Arabidopsis) (COPS2))

Alternative Name: COPS2 / TRIP15 (COPS2 Products)

Synonyms: COPS2 antibody, ALIEN antibody, CSN2 antibody, SGN2 antibody, TRIP15 antibody, wu:fc05f05 antibody, wu:fc06g10 antibody, wu:fc60f04 antibody, wu:fl94f12 antibody, zgc:86624 antibody, csn2 antibody, Tb03.48O8.600 antibody, Trip15 antibody, AI315723 antibody, C85265 antibody, Csn2 antibody, Sgn2 antibody, alien-like antibody, ATCSN2 antibody, CONSTITUTIVE PHOTOMORPHOGENIC 12 antibody, COP12 antibody, FUSCA 12 antibody, T20P8.4 antibody, T20P8_4 antibody, COP9 signalosome subunit 2 antibody, COP9 signalosome subunit 2 L homeolog antibody, COP9 signalosome complex subunit 2 antibody, COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis) antibody, proteasome family protein antibody, COPS2 antibody, cops2 antibody, cops2.L antibody, Tb927.3.2320 antibody, AOR_1_576084 antibody, PTRG_07106 antibody, LOC100283498 antibody, Cops2 antibody, csn-2 antibody, FUS12 antibody

Background: TRIP15 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP. TRIP15 is also involved in early stage of neuronal differentiation via its interaction with NIF3L1.Synonyms: Alien homolog, COP9 signalosome complex subunit 2, CSN2, JAB1-containing signalosome subunit 2, SGN2, TRIP-15, Thyroid receptor-interacting protein 15

Gene ID: 9318

NCBI Accession: NP_004227

UniProt: P61201

Pathways: Cell Division Cycle