NUP50 antibody (C-Term)

Catalog No. ABIN374317

Product Details anti-NUP50 Antibody

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This NUP50 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-ELHKILLEKK DA

Specificity: This antibody detects NUP50 at C-term. It is expected to recognise both human isoforms of this protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_009103.2, NP_705931.1. Genename: NUP50

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 60 kDa band seen in Jurkat lysate (predicted MW 54 kDaaccording to NP_009103).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NUP50

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Alternative Name: NUP50 (NUP50 Products)

Background: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. It interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1.Synonyms: NPAP60L, Nuclear pore-associated protein 60 kDa-like, Nucleoporin 50 kDa

Gene ID: 10762

NCBI Accession: NP_009103

UniProt: Q9UKX7

Pathways: Tube Formation