NEIL1 antibody (C-Term)

Catalog No. ABIN374304

Product Details anti-NEIL1 Antibody

Target: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NEIL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-DIPSLEPEGT SAS

Specificity: This antibody detects NEIL1 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_078884.2 Genename: NEIL1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: Experiments gave an approx 60 kDa band in Human Liver and Brain lysates at1 μg/mL. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Placenta shows strongstaining of cytoplasm in syncytiotrophoblasts.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for NEIL1

Target: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Alternative Name: NEIL1 (NEIL1 Products)

Background: Human NEIL1 is the homologs of Escherichia coli Nei. Human NEIL1 is a DNA glycosylase, that phylogenetically belongs to the Fpg/Nei family, primarily found in the bacterial kingdom and is functionally homologous to bacterial Nei. Thymine glycol, a potentially lethal DNA lesion produced by reactive oxygen species, can be removed by DNA glycosylases. The NEIL1 gene is located on the long arm of chromosome 15 that is frequently deleted in human cancers.Synonyms: DNA glycosylase/AP lyase Neil1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Endonuclease VIII-like 1, FPG1, NEH1

Gene ID: 79661

NCBI Accession: NP_078884

UniProt: Q96FI4

Pathways: DNA Damage Repair