MXD4 antibody (C-Term)

Catalog No. ABIN374270

Product Details anti-MXD4 Antibody

Target: MXD4 (MAX Dimerization Protein 4 (MXD4))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MXD4 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GPHCRRLGRP ALS

Specificity: This antibody detects MAD4 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_006445.1 Genename: MXD4

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.5-2 μg/mL. Approx 22 kDa band observed in human kidney lysates(predicted MW of 22 kDa according to NP_006445). Please note the appearance of thisband was blocked by incubation with the immunising peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for MXD4

Target: MXD4 (MAX Dimerization Protein 4 (MXD4))

Alternative Name: MAD4 (MXD4 Products)

Background: MAD4 is a member of the MAD gene family . The MAD genes encode basic helix loop helix leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues.Synonyms: MAD-4, MAX dimerization protein 4, MXD4, Max-associated protein 4, Max-interacting transcriptional repressor MAD4

Gene ID: 10608

NCBI Accession: NP_006445

UniProt: Q14582