IPO7 antibody (C-Term)

Catalog No. ABIN374249

Product Details anti-IPO7 Antibody

Target: IPO7 (Importin 7 (IPO7))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Cow, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This IPO7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-SSFNFGGPAP GMN

Specificity: This antibody detects RanBP7/Importin-7 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic peptide from C-terminus of Human IPO7 (NP_006382.1). Genename: IPO7

Application Details

Application Notes: Peptide ELISA: Limit dilution: 1/32000. Western blot: 0.1-1 μg/mL. Approx 120 kDa band observed in Human Brain lysates(predicted MW of 110 kDa according to NP_006382.1). Please note that an approx 30 kDaband of unknown identity is also consistently observed. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing of 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IPO7

Target: IPO7 (Importin 7 (IPO7))

Alternative Name: RanBP7 / Importin-7 (IPO7 Products)

Background: The Importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran binding site of importin beta. Similar to importin beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta like transport receptors, namely importin beta itself, transportin, RanBP5 and Importin 7, directly bind and import ribosomal proteins.Synonyms: IMP7, IPO7, Ran-binding protein 7

Gene ID: 10527

NCBI Accession: NP_006382

UniProt: O95373

Pathways: Protein targeting to Nucleus