AKR1A1 antibody (C-Term)

Catalog No. ABIN374244

Product Details anti-AKR1A1 Antibody

Target: AKR1A1 (Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase) (AKR1A1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This AKR1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-DAGHPLYPFN DPY

Specificity: This antibody detects AKR1A1 / ALDR1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_006057.1, NP_697021.1. Genename: AKR1A1

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: Approx 38 kDa band observed in Human Placenta and Liver lysates(calculated MW of 36.8 kDa according to NP_006057.1). Recommended concentration: 0.5 - 2 μg/mL. Immunohistochemistry on paraffin sections: Human Kidney shows Brush Border stainingin PCT. Recommended concentration: 2 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for AKR1A1

Target: AKR1A1 (Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase) (AKR1A1))

Alternative Name: AKR1A1 / ALDR1 (AKR1A1 Products)

Background: The AKR1A1 gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Alternative splicing of this gene results in two transcript variants encoding the same protein.Synonyms: ALR, Alcohol dehydrogenase [NADP+], Aldehyde reductase, Aldo-keto reductase family 1 member A1

Gene ID: 10327

NCBI Accession: NP_006057

UniProt: P14550

Pathways: Monocarboxylic Acid Catabolic Process