ARPC1B antibody (N-Term)

Catalog No. ABIN374216

Product Details anti-ARPC1B Antibody

Target: ARPC1B (Actin Related Protein 2/3 Complex, Subunit 1B, 41kDa (ARPC1B))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This ARPC1B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AYHSFLVEPI SCH

Specificity: This antibody detects ARPC1B / ARC41 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_005711.1 Genename: ARPC1B

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 2-4 μg/mL. Approx 37 kDa band observed in Human Liver lysates (calculatedMW of 40.7 kDa according to NP_005711). A minor band of unknown identity was alsoconsistently observed at 27 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for ARPC1B

Target: ARPC1B (Actin Related Protein 2/3 Complex, Subunit 1B, 41kDa (ARPC1B))

Alternative Name: ARPC1B / ARC41 (ARPC1B Products)

Background: ARP2/3 subunit 1B (ARPC1B) is one of seven subunits of the human Arp2/3 protein complex. It is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages.Synonyms: Actin-related protein 2/3 complex subunit 1B, Arp2/3 complex 41 kDa subunit, p41-ARC

Gene ID: 10095

NCBI Accession: NP_005711

UniProt: O15143

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization