ARPC2 antibody (C-Term)

Catalog No. ABIN374215

Product Details anti-ARPC2 Antibody

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ARPC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-EMKTITGKTF SSR

Specificity: This antibody detects ARPC2 / ARC34 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and CowSpecies reactivity (tested):Human

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_005722.1, NP_690601.1. Genename: ARPC2

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.1-0.5 μg/mL. Approx 33-34 kDa band observed in Hela and Human SkeletalMuscle lysates (predicted MW of 34 kDa according to NP_005722). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Spleen shows cytoplasmstaining in some splenocytes in and around a T-cell area.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ARPC2

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Alternative Name: ARPC2 / ARC34 (ARPC2 Products)

Background: ARPC2 is one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of ARPC2, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.Synonyms: Actin-related protein 2/3 complex subunit 2, Arp2/3 complex 34 kDa subunit, PRO2446, p34-ARC

Gene ID: 10109

NCBI Accession: NP_005722

UniProt: O15144

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization