TFEB antibody (N-Term)

Catalog No. ABIN374208

Product Details anti-TFEB Antibody

Target: TFEB (Transcription Factor EB (TFEB))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This TFEB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Chromatin Immunoprecipitation (ChIP)

Sequence: ASRIGLRMQL MRE-C

Specificity: This antibody detects TFEB at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Porcine and Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_009093.1 Genename: TFEB

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.5-2 μg/mL. Approx 50-55 kDa band seen in NIH-3T3 lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TFEB

Target: TFEB (Transcription Factor EB (TFEB))

Alternative Name: TFEB (TFEB Products)

Background: Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.Synonyms: Transcription factor EB

Gene ID: 7942

NCBI Accession: NP_009093

UniProt: P19484