ADAM8 antibody (C-Term)

Catalog No. ABIN374195

Product Details anti-ADAM8 Antibody

Target: ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ADAM8 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-QRKQGAGAPT AP

Specificity: This antibody is expected to recognise isoform 1 and 3 (NP_001100.3 and NP_001157962.1).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_001100.3, NP_001157962.1. Genename: ADAM8

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 1000. Western blot: 1-3 μg/mL. Approx 90 kDa band seen in Human Brain lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ADAM8

Target: ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))

Alternative Name: CD156a / ADAM8 (ADAM8 Products)

Background: Members of ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. The extracellular region of ADAM8 shows significant amino acid sequence homology to hemorrhagic snake venom proteins, including the metalloprotease and disintegrin domains. The expression of ADAM8 is upregulated by retinoic acid and vitamin D3. The function of ADAM8 is still poorly understood. ADAM8 has been shown to stimulate osteoclasts, and to be produced by bone marrow cultures stimulated by vitamin-D. ADAM8 is involved in protein processing at the cell surface and is also thought to act as a leukocyte differentiation marker.Synonyms: Cell surface antigen MS2, Disintegrin and metalloproteinase domain-containing protein 8, MS2

Molecular Weight: 88.8 kDa according to NP_001100.3.

Gene ID: 101

NCBI Accession: NP_001100

UniProt: P78325

Pathways: Activation of Innate immune Response, M Phase