Axin antibody (C-Term)

Catalog No. ABIN374189

Product Details anti-Axin Antibody

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Axin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-FEEKIIGKVE KVD

Specificity: This antibody detects Axin-1 at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_003493.1, NP_851393.1. Genename: AXIN1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 128000. Western blot: Preliminary experiments gave bands at approx 150 kDa and 22-20 kDa inHuman Brain (Hippocampus) lysates after 0.1 μg/mL antibody staining. Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Adrenal Gland showsmembranous and fibrous staniing in and around secretory cells of the zona fasciculata inthe cortex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for Axin

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Alternative Name: Axin-1 (AXIN1 Products)

Background: Axin 1 is a negative regulator of Wnt signaling and is also associated with cancer. Axin 1 contains RGS (Regulator of G protein signaling domain) and Axin_b-cat_bind (Axin beta-catenin binding domain).Synonyms: AXIN, AXIN1, Axis inhibition protein 1

Gene ID: 8312

NCBI Accession: NP_003493

UniProt: O15169

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling