TMPRSS5
Reactivity: Human
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 647
Application Notes
Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-1.5 μg/mL. Experiments gave bands at approx 50 kDa and 20 kDa inHuman Brain (Cerebral Cortex) lysates and in Mouse and Rat Brain lysates after 0.5 μg/mLantibody staining. These bands correspond to earlier findings in literature with differentantibodies (Yamaguchi et al, J Biol Chem.2002 Mar 1,277(9): 6806-12, PMID: 11741986). This protein has a calculated MW of 49.0 kDa according to NP_110397.1. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.