VAV2 antibody (Internal Region)

Catalog No. ABIN374176

Product Details anti-VAV2 Antibody

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This VAV2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: ETEAKYYRTL EDIEC

Specificity: This antobody is expected to recognise both reported isoforms of Human VAV2 (NP_001127870.1 and NP_003362.2).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, and Pig.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from internal region of the protein sequence according to NP_001127870.1, NP_003362.2. Genename: VAV2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-1.5 μg/mL. Approx 100 kDa band observed in 293 lysate (predicted MW of100 kDa according to NP_003362).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for VAV2

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Alternative Name: VAV2 (VAV2 Products)

Background: VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.Synonyms: Guanine nucleotide exchange factor VAV2, VAV-2

Gene ID: 7410

NCBI Accession: NP_001127870

UniProt: P52735

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway