ELMO1 antibody (C-Term)

Catalog No. ABIN374159

Product Details anti-ELMO1 Antibody

Target: ELMO1 (Engulfment and Cell Motility 1 (ELMO1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ELMO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: PKEPSNYDFV YDCN

Specificity: This antibody is expected to recognise both Human isoforms of this protein (NP_569709.1 and NP_001034548.1 represent the same isoform).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human, Bovine, Zebrafish.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_055615.8, NP_569709.1, NP_001034548.1 Genename: ELMO1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: Recommended for use at 1-2 μg/mL. Approx 70 kDa and 50 kDa bands seen inJurkat cell lysate. Note the smaller isoform of this protein has a consensus glycosylationsite, which may explain the higher than expected band size (50 kDa versus 30 kDa). Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B. D and Rickwood, D. ,eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-ELMO1 antibody (ABIN374159)

Epting, Slanchev, Boehlke, Hoff, Loges, Yasunaga, Indorf, Nestel, Lienkamp, Omran, Kuehn, Ronneberger, Walz, Kramer-Zucker: "The Rac1 regulator ELMO controls basal body migration and docking in multiciliated cells through interaction with Ezrin." in: Development (Cambridge, England), Vol. 142, Issue 1, pp. 174-84, (2014) (PubMed).

Petrosyan, Ali, Verma, Cheng, Cheng: "Non-muscle myosin IIA transports a Golgi glycosyltransferase to the endoplasmic reticulum by binding to its cytoplasmic tail." in: The international journal of biochemistry & cell biology, Vol. 44, Issue 7, pp. 1153-65, (2012) (PubMed).

Huenniger, Krämer, Soom, Chang, Köhler, Depping, Kehlenbach, Kaether: "Notch1 signaling is mediated by importins alpha 3, 4, and 7." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 18, pp. 3187-96, (2010) (PubMed).

Vassilieva, Gerner-Smidt, Ivanov, Nusrat: "Lipid rafts mediate internalization of beta1-integrin in migrating intestinal epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 5, pp. G965-76, (2008) (PubMed).

Target Details for ELMO1

Target: ELMO1 (Engulfment and Cell Motility 1 (ELMO1))

Alternative Name: ELMO1 (ELMO1 Products)

Background: ELMO1 is involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. ELMO1 acts in association with DOCK1 and CRK and was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. It is belived to enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. ELMO1 interacts directly with the SH3-domain of DOCK1 via its SH3-binding site. It is part of a complex with DOCK1 and RAC1 and with DOCK1 and CRK isoform CRK-II. Recent studies show that it interacts with PLEKHG6.Synonyms: CED-12 homolog, ELMO 1, ELMO-1, Engulfment and cell motility protein 1, KIAA0281

Gene ID: 9844

NCBI Accession: NP_055615

UniProt: Q92556