DOK3 antibody (C-Term)

Catalog No. ABIN374125

Product Details anti-DOK3 Antibody

Target: DOK3 (Docking Protein 3 (DOK3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This DOK3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TRERRKGPAP CDRP

Specificity: This antibody detects Docking protein 3/DOK3 (C-term).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from C Terminus of the protein sequence according to NP_079148.2 Genename: DOK3

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/64000. Western blot: 0.1-0.3 μg/mL. Approx 60 kDa band observed in Human Peripheral BloodMononucleocytes lysates (calculated MW of 53.0 kDa according to NP_079148.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DOK3

Target: DOK3 (Docking Protein 3 (DOK3))

Alternative Name: DOK3 (DOK3 Products)

Background: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl functionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function. There are 4 isoforms generated by alternative splicing.Synonyms: Docking protein 3, Downstream of tyrosine kinase 3

Gene ID: 79930

NCBI Accession: NP_079148

UniProt: Q7L591