SOCS1 antibody (C-Term)

Catalog No. ABIN374072

Product Details anti-SOCS1 Antibody

Target: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This SOCS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-VLRDYLSSFP FQI

Specificity: Recognizes Suppressor Of Cytokine Signaling 1 (SOCS1) at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog) and Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic peptide from the C-Terminus of Human SOCS1 (NP_003736.1). Genename: SOCS1

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/4000. Western blot: 1-3 μg/mL. In transfected HEK293 transiently expressing human SOCS1 bandsof approx.30 kDa and 20 kDa are observed. These bands are not observed in thenon-transfected HEK293. A similar pattern is observed in Mouse Spleen lysates. Thecalculated molecular size is 23.0 kDa according to Human NP_003736.1 and 23.0 kDa

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline buffer, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SOCS1

Target: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Alternative Name: SOCS1 (SOCS1 Products)

Background: SOCS1 represents a family of proteins that regulates responses to cytokines. The SOCS family comprises at least 8 members, SOCS1 to SOCS7 and CIS (cytokine inducible SH2 containing protein). Structurally, the SOCS proteins are composed of an N terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C terminal motif named the SOCS box. SOCS1 (also known as Janus kinase binding protein and STAT induced STAT inhibitor 1) was isolated independently by its ability to interact with Janus kinase 2 in a 2 hybrid screen and on the basis of antigenic similarity with the SH2 domain of STAT3. SOCS 1 inhibition of signal transduction appears to occur by binding to and inhibiting the catalytic activity of members of JAK family of cytoplasmic kinases (direct JAK2 kinase inhibition). Biochemical characterization as well as gene disruption studies indicate that SOCS1 is an important negative regulator of JAK-STAT signal pathway.Synonyms: JAB, JAK-binding protein, SOCS-1, SSI-1, SSI1, STAT-induced STAT inhibitor 1, Suppressor of cytokine signaling 1, TIP-3, TIP3, Tec-interacting protein 3

Gene ID: 8651

NCBI Accession: NP_003736

UniProt: O15524

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, TLR Signaling, Response to Growth Hormone Stimulus