Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Aurora A antibody (C-Term)

AURKA Reactivity: Human WB Host: Goat Polyclonal unconjugated
Catalog No. ABIN374071
  • Target See all Aurora A (AURKA) Antibodies
    Aurora A (AURKA) (Aurora Kinase A (AURKA))
    Binding Specificity
    • 22
    • 11
    • 9
    • 8
    • 8
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 112
    • 66
    • 37
    • 21
    • 18
    • 12
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    Human
    Host
    • 107
    • 17
    • 1
    Goat
    Clonality
    • 100
    • 24
    Polyclonal
    Conjugate
    • 76
    • 8
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Aurora A antibody is un-conjugated
    Application
    • 90
    • 48
    • 32
    • 24
    • 15
    • 11
    • 10
    • 7
    • 7
    • 3
    • 2
    • 1
    Western Blotting (WB)
    Sequence
    C-QNKESASKQS
    Specificity
    This antibody detects Aurora kinase A / AURKA at C-term.
    Purification
    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
    Immunogen
    Peptide from the C Terminus of the protein sequence according to NP_003591.2.
    Top Product
    Discover our top product AURKA Primary Antibody
  • Application Notes
    Peptide ELISA: Detection limit dilution 1: 128000. Western blot: 0.1-0.3 μg/mL. Approx 45 kDa band observed in lysates of cell line HeLa andJurkat lysate (calculated MW of 45.5 kDa according to NP_003591.2). In transfected HEK293transiently expressing AURKA a band of approx.45 kDa is observed. This band is notobserved in the non-transfected HEK293.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
  • Target
    Aurora A (AURKA) (Aurora Kinase A (AURKA))
    Alternative Name
    Aurora Kinase A (AURKA Products)
    Background
    Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Synonyms: AIK1, ARK1, AURA, AURKA, AURORA2, Aurora/IPL1-related kinase 1, BTAK, Breast tumor-amplified kinase, STK15, STK6, Serine/threonine kinase 15, Serine/threonine-protein kinase 6
    Gene ID
    6790
    NCBI Accession
    NP_003591
    UniProt
    O14965
    Pathways
    Cell Division Cycle, Asymmetric Protein Localization
You are here:
Support