GADD34 antibody (C-Term)
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- Target See all GADD34 (PPP1R15A) Antibodies
- GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This GADD34 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Sequence
- C-AAALDLSGRR G
- Specificity
- This antibody detects GADD34 / PPP1R15A at C-term.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human, Mouse.
- Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- Immunogen
- Peptide from the C Terminus of the protein according to NP_055145.3. Genename: PPP1R15A
- Top Product
- Discover our top product PPP1R15A Primary Antibody
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anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (C-Term) antibody
Verified PPP1R15A Reactivity: Human WB, ELISA, IHC, FACS, IF Host: Goat Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) antibodyPPP1R15A Reactivity: Human, Mouse, Rat, Monkey, Dog WB, IHC, FACS, IF, IHC (p) Host: Mouse Monoclonal 3D12 unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 375-674) antibodyPPP1R15A Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 401-500) antibodyPPP1R15A Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IF (cc), IF (p), IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 430-490) antibodyPPP1R15A Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 401-500) antibody (AbBy Fluor® 647)PPP1R15A Reactivity: Human, Mouse, Rat WB, FACS, IF (cc), IF (p) Host: Rabbit Polyclonal AbBy Fluor® 647
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 116-165) antibodyPPP1R15A Reactivity: Human, Monkey WB Host: Rabbit Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 433-636), (Regulatory Subunit 15A) antibodyPPP1R15A Reactivity: Mouse WB, IHC, ICC Host: Rabbit Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 335-578), (Regulatory Subunit 15A) antibodyPPP1R15A Reactivity: Rat WB, IHC, ICC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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ELISA: 1: 128000. Western blot: 0.1-0.3 μg/mL. Approx 75 kDa band observed in lysates of cell line HEPG2(calculated MW of 73.5 kDa according to NP_055145.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))
- Alternative Name
- GADD34 / PPP1R15A (PPP1R15A Products)
- Background
- A protein phosphatase whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents.Synonyms: Growth arrest and DNA damage-inducible protein GADD34, Myeloid differentiation primary response protein MyD116 homolog, Protein phosphatase 1 regulatory subunit 15A
- Gene ID
- 23645
- NCBI Accession
- NP_055145
- UniProt
- O75807
- Pathways
- ER-Nucleus Signaling, Unfolded Protein Response
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