PGAM1 antibody (C-Term)

Catalog No. ABIN374067

Product Details anti-PGAM1 Antibody

Target: PGAM1 (phosphoglycerate Mutase 1 (Brain) (PGAM1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This PGAM1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-KAMEAVAAQG KAKK

Specificity: This antibody detects PGAM1/PGAM2/PGAM4 at C-term. It is expected to recognize the products of 3 highly similar genes.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_002620.1, NP_000281.2, NP_001025062.1. Genename: PGAM1 / PGAM2 / PGAM4

Application Details

Application Notes: ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 28 kDa band observed in human liver and brain lysates(predicted MW of 28 kDa according to NP_002620).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PGAM1

Target: PGAM1 (phosphoglycerate Mutase 1 (Brain) (PGAM1))

Alternative Name: PGAM1 (PGAM1 Products)

Background: Synonyms: PGAM 1, PGAM 2, PGAM 4, PGAM-B, PGAM-M, PGAM3, PGAMA, PGAMM, Phosphoglycerate mutase 1, Phosphoglycerate mutase 2, Probable phosphoglycerate mutase 4

Gene ID: 5223

NCBI Accession: NP_002620

UniProt: P18669

Pathways: Regulation of Carbohydrate Metabolic Process