Axin antibody (C-Term)

Catalog No. ABIN372934

Product Details anti-Axin Antibody

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Axin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: c-FEEKIIGKVE KVD

Specificity: This antibody recognizes Axin 1 (AXIN1).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide from C-terminus of Human AXIN1.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/28000. Immunohistochemistry on Paraffin Sections: 3.75 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris-Saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Axin

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Alternative Name: Axin-1 (AXIN1 Products)

Background: Axin-1 (Axis inhibition protein 1) and Axin-2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin-1 interacts with APC, GSK3-beta, Dvl and beta-Catenin and promotes the GSK3-beta-mediated phosphorylation and subsequent degradation of beta-Catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute Axin1 in mice (6). Axin-2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).Synonyms: AXIN, AXIN1, Axis inhibition protein 1

Gene ID: 8312

NCBI Accession: NP_003493

UniProt: O15169

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling