SYT1 antibody

Catalog No. ABIN372723

Product Details anti-SYT1 Antibody

Target: SYT1 (Synaptotagmin I (SYT1))

Reactivity: Human, Rat, Mouse, Cow, Chicken, Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYT1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody recognizes the ~65k Synaptotagmin I protein.

Cross-Reactivity (Details): Species reactivity (expected):Human, Mouse, Bovine, Chicken and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acid residues within the Synaptotagmin-1 protein.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: Synaptotagmin-1 (SYT1 Products)

Background: Synaptotagmin-1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release (Littleton and Bellen 1995). Recent studies indicate that Synaptotagmin's Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane fusion (Zhang et al., 2002). It has recently been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size (Poskanzer et al., 2006).Synonyms: SVP65, SYT, SYT1, Synaptotagmin I, Synaptotagmin1, SytI, p65

Gene ID: 6857

NCBI Accession: NP_001129277

UniProt: P21579

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport