SYN1 antibody (pSer603)

Catalog No. ABIN372721

Product Details anti-SYN1 Antibody

Target: SYN1 (Synapsin I (SYN1))

Binding Specificity: pSer603

Reactivity: Human, Mouse, Rat, Cow, Xenopus laevis, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYN1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody recognizes ~78k Synapsin I doublet protein phosphorylated at Ser603. Immunolabeling of the synapsin I band is blocked by lambda-phosphatase treatment.

Cross-Reactivity (Details): Species reactivity (expected):Human, Bovine, Mouse, Xenopus and Zebrafish.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser603 of Synapsin I.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for SYN1

Target: SYN1 (Synapsin I (SYN1))

Alternative Name: Synapsin-1 (SYN1 Products)

Background: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002, Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001, Kao et al., 2002). Serine 603 is the site on synapsin I that is phosphorylated by calcium calmodulin kinase II and by p21-activated kinases (Sakurada et al., 2002, Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function (Nayak et al., 1996, Bahler and Greengard, 1987, McGuinness et al., 1989).Synonyms: Brain protein 4.1, SYN1, Synapsin I

Gene ID: 6853

NCBI Accession: NP_008881

UniProt: P17600