SYN1 antibody (pSer62, pSer67)

Catalog No. ABIN372719

Product Details anti-SYN1 Antibody

Target: SYN1 (Synapsin I (SYN1))

Binding Specificity: pSer62, pSer67

Reactivity: Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYN1 antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Specificity: This antibody recognizes the ~78k Synapsin I doublet phosphorylated at Ser62,67. Immunolabeling of the Synapsin I band is blocked by preadsorption with the phospho-peptide used as antigen but not be the corresponding dephospho-peptide.

Cross-Reactivity (Details): Species reactivity (expected):Bovine (100 %) and Mouse (100 %).
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the Phospho Serine 62,67 of Synapsin I.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, None, 100 μg/mL BSA and 50 % Glycerol

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-SYN1 antibody (ABIN372719)

Tallent, Varghis, Skorobogatko, Hernandez-Cuebas, Whelan, Vocadlo, Vosseller: "In vivo modulation of O-GlcNAc levels regulates hippocampal synaptic plasticity through interplay with phosphorylation." in: The Journal of biological chemistry, Vol. 284, Issue 1, pp. 174-81, (2008) (PubMed).

Target Details for SYN1

Target: SYN1 (Synapsin I (SYN1))

Alternative Name: Synapsin-1 (SYN1 Products)

Background: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002, Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001, Kao et al., 2002). Ser 549 along with Ser 62 and Ser 67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Czernik et al., 1987, Jovanovic et al., 1996).Synonyms: Brain protein 4.1, SYN1, Synapsin I

Gene ID: 6853

NCBI Accession: NP_008881

UniProt: P17600