Periostin antibody

Catalog No. ABIN372701

Product Details anti-Periostin Antibody

Target: Periostin (POSTN)

Reactivity: Human, Mouse, Rat, Chicken, Mammalian, Amphibian, Fish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Periostin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognizes the three Periostin bands around ~93 kDa in Mouse lung extract. The immunogenic peptide is present in all known splice variants of Periostin and therefore this antibody recognizes all known molecular forms of Periostin.

Cross-Reactivity (Details): Species reactivity (expected):Mammalia, Birds, Amphibians and Fishes.
Species reactivity (tested):Human, Chicken, Mouse and Rat.

Purification: Affinity Chromatography.

Immunogen: Peptide from fasciclin domain 1 of Mouse Periostin.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry: 1/100. Immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrievalprotocol (incubate slides for 20 minutes at 90 °C in 10 mM sodium citrate ( pH 6.0)/ 0.1 % Tween-20).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C. For long term store the antibody (in aliquots) at -80 °C.

Target Details for Periostin

Target: Periostin (POSTN)

Alternative Name: Periostin (POSTN Products)

Background: Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has recently been shown to promote collagen fibrogenesis, inhibit differentiation of progenitor cells into cardiomyocytes and to be essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008)Synonyms: OSF-2, OSF2, Osteoblast-specific factor 2, PN, POSTN

Gene ID: 10631

NCBI Accession: NP_001129406

UniProt: Q15063