Parkin antibody (pSer101)
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- Target See all Parkin (PARK2) Antibodies
- Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))
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Binding Specificity
- pSer101
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Reactivity
- Human, Cow, Non-Human Primate
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Parkin antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- This antibody recognizes the ~52k Parkin protein phosphorylated at Ser101. Immunolabeling of the parkin band is absent in parkin S101 mutants.
- Cross-Reactivity (Details)
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Species reactivity (expected):Bovine and non-Human Primates.
Species reactivity (tested):Human. - Purification
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
- Immunogen
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser101 of human Parkin.
- Isotype
- IgG
- Top Product
- Discover our top product PARK2 Primary Antibody
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anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 387-417), (C-Term) antibody
PARK2 Reactivity: Human, Mouse WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB07373 unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 288-387) antibodyPARK2 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1H4 unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-300) antibodyPARK2 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 387-417) antibodyPARK2 Reactivity: Human, Mouse WB, ELISA, IHC, IF, FACS Host: Rabbit Polyclonal unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 111-140) antibodyPARK2 Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Rabbit Polyclonal unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-465) antibodyPARK2 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 361-465) antibodyPARK2 Reactivity: Rat WB, ELISA, IF (cc), IF (p), IHC (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-144) antibodyPARK2 Reactivity: Human ELISA, FACS Host: Mouse Monoclonal 6F5D2 unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 23-416) antibodyPARK2 Reactivity: Human, Mouse, Rat WB, IHC (p), ICC Host: Rabbit Polyclonal unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 111-140), (N-Term) antibodyPARK2 Reactivity: Human WB Host: Rabbit Polyclonal RB7371 unconjugated
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- Application Notes
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Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the antibody undiluted (in aliquots) at-20 °C.
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- Target
- Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))
- Alternative Name
- PARK2 / Parkin (PARK2 Products)
- Background
- Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson's disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (Jenner et al.,1992). Recent evidence suggests that phosphorylation of parkin at Ser101 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005).Synonyms: AR-JP, E3 ubiquitin-protein ligase parkin, LPRS2, PDJ, PRKN, Parkinson disease 2, Parkinson juvenile disease protein 2
- Gene ID
- 5071
- NCBI Accession
- NP_004553
- UniProt
- O60260
- Pathways
- Autophagy, Ubiquitin Proteasome Pathway
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