MAP2K5 antibody (pSer311, pThr315)

Catalog No. ABIN372667

Product Details anti-MAP2K5 Antibody

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Binding Specificity: pSer311, pThr315

Reactivity: Human, Mouse, Rat, Dog, Cow, Xenopus laevis, Zebrafish (Danio rerio), Chicken, Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K5 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody is specific for the ~49k MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling of the MEK5 band is blocked by preadsorption with the phospho-peptide used as antigen but not be the corresponding dephospho-peptide.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Human, Chicken, Bovine, Canine, non-Human Primates, Xenopus, and Zebrafish.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser311, Thr315 of MEK5.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for MAP2K5

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Alternative Name: MAP2K5 (MAP2K5 Products)

Background: MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation (Cameron et al., 2004).Synonyms: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MEK5, MKK5

Gene ID: 29568

NCBI Accession: NP_001029159

UniProt: Q62862

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway