DARPP32 antibody (N-Term)

Catalog No. ABIN372605

Product Details anti-DARPP32 Antibody

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Chicken, Xenopus laevis, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DARPP32 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Specific for the ~32k DARPP-32 protein.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, Xenopus and Zebrafish.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogen: Peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000. Immunohistochemistry: 1/250.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-DARPP32 antibody (ABIN372605)

Bonito-Oliva, DuPont, Madjid, Ögren, Fisone: "Involvement of the Striatal Medium Spiny Neurons of the Direct Pathway in the Motor Stimulant Effects of Phencyclidine." in: The international journal of neuropsychopharmacology, (2016) (PubMed).

Gulati, Cheung, Antrobus, Church, Harding, Tung, Rimmington, Ma, Ron, Lehner, Ashcroft, Cox, Coll, ORahilly, Yeo: "Role for the obesity-related FTO gene in the cellular sensing of amino acids." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 7, pp. 2557-62, (2013) (PubMed).

Rewal, Jurd, Gill, He, Ron, Janak: "Alpha4-containing GABAA receptors in the nucleus accumbens mediate moderate intake of alcohol." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 2, pp. 543-9, (2009) (PubMed).

Haycock, Becker, Ang, Furukawa, Hornykiewicz, Kish: "Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum." in: Journal of neurochemistry, Vol. 87, Issue 3, pp. 574-85, (2003) (PubMed).

Waymire, Haycock: "Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells." in: Journal of neurochemistry, Vol. 81, Issue 3, pp. 589-93, (2002) (PubMed).

Kish, Kalasinsky, Derkach, Schmunk, Guttman, Ang, Adams, Furukawa, Haycock: "Striatal dopaminergic and serotonergic markers in human heroin users." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 24, Issue 5, pp. 561-7, (2001) (PubMed).

Zhu, Klimek, Haycock, Ordway: "Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain." in: Journal of neuroscience methods, Vol. 99, Issue 1-2, pp. 37-44, (2000) (PubMed).

Zhu, Klimek, Dilley, Haycock, Stockmeier, Overholser, Meltzer, Ordway: "Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression." in: Biological psychiatry, Vol. 46, Issue 9, pp. 1275-86, (1999) (PubMed).

Target Details for DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Alternative Name: DARPP32 (PPP1R1B Products)

Background: DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002).Synonyms: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B

Gene ID: 84152

NCBI Accession: NP_001229393

UniProt: Q9UD71