CDK1 antibody (pTyr15)

Catalog No. ABIN372596

Product Details anti-CDK1 Antibody

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Binding Specificity: pTyr15

Reactivity: Human, Mouse, Rat, Xenopus laevis, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for the ~38k CDC2 protein phosphorylated at Tyr15. Immunolabeling is blocked by the lambda-phosphatase treatment.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Zebrafish.
Species reactivity (tested):Human and Xenopus.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr15 of rat CDC2.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDK1 (CDK1 Products)

Background: Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle (Maller, 1991). The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2 (Nakamizo et al., 2002). cdc2 expression in brain has been linked to both neurogenesis and apoptosis (Konishi and Bonni, 2003, Dranovsky et al., 2001, Okano et al., 1996).Synonyms: CDC2, CDC28A, CDK1, CDKN1, Cell division control protein 2 homolog, Cell division protein kinase 1, Cyclin-dependent kinase 1, P34CDC2, p34 protein kinase

Gene ID: 983

NCBI Accession: NP_001777

UniProt: P06493

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA