BU-1A / B6.1 antibody

Catalog No. ABIN371411

Product Details anti-BU-1A / B6.1 Antibody

Target: BU-1A / B6.1

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This BU-1A / B6.1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognizes Chicken Bu-1a alloantigen (Mr. 70 kDa). It does not react with cells from CHA and H.B15 strains by Immunofluorescence.

Characteristics: Synonyms: B-Cell Marker, BU-1 Allele A

Purification: Purified

Immunogen: Bursal cells from one-day-old H. B15 (Bu-1a/b) strain chickens.

Clone: 21-1A4

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells. Immunoprecipitation. Immunohistochemistry on Frozen Sections. (Ref.3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for BU-1A / B6.1

Target: BU-1A / B6.1

Abstract: BU-1A / B6.1 Products

Background: Chicken Bu-1a, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90 % of bursal cells, 2-8 % of thymocytes, 15-27 % of spleen cells, and 2-18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In Chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion.Synonyms: B-Cell Marker, BU-1 Allele A

Gene ID: 396098

UniProt: Q90746