CD28 antibody

Catalog No. ABIN370901

Product Details anti-CD28 Antibody

Target: CD28

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Characteristics: Synonyms: TP44, T-cell-specific surface glycoprotein CD28

Purification: Purified

Clone: 37-51

Isotype: IgG

Application Details

Application Notes: Flow Cytometry. (Ref.2-5)Immunoprecipitation. (Ref.2)Functional Assay: In vitro costimulation of T cells and NK cells. (Ref.1,4,7)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-CD28 antibody (ABIN370901)

Shen, Okwan-Duodu, Blackwell, Ong, Janjulia, Bernstein, Fuchs, Alkan, Bernstein: "Myeloid expression of angiotensin-converting enzyme facilitates myeloid maturation and inhibits the development of myeloid-derived suppressor cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 94, Issue 5, pp. 536-44, (2014) (PubMed).

Belhacéne, Gamas, Gonçalvès, Jacquin, Beneteau, Jacquel, Colosetti, Ricci, Wakkach, Auberger, Marchetti: "Severe thymic atrophy in a mouse model of skin inflammation accounts for impaired TNFR1 signaling." in: PLoS ONE, Vol. 7, Issue 10, pp. e47321, (2012) (PubMed).

Kim, Sullivan, Plisch, Tejera, Jatzek, Choi, Suresh: "Signal integration by Akt regulates CD8 T cell effector and memory differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 9, pp. 4305-14, (2012) (PubMed).

Silverio, Pereira, Cipitelli, Vinagre, Rodrigues, Gazzinelli, Lannes-Vieira: "CD8+ T-cells expressing interferon gamma or perforin play antagonistic roles in heart injury in experimental Trypanosoma cruzi-elicited cardiomyopathy." in: PLoS pathogens, Vol. 8, Issue 4, pp. e1002645, (2012) (PubMed).

Zhang, Fresnay, Welty, Sangrampurkar, Rybak, Zhou, Cheng, Feng, Avon, Laaris, Whitters, Nagelin, OHara, Azimzadeh: "Selective CD28 blockade attenuates acute and chronic rejection of murine cardiac allografts in a CTLA-4-dependent manner." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 11, Issue 8, pp. 1599-609, (2011) (PubMed).

Lin, Harding, Giurisato, Shaw: "KSR1 modulates the sensitivity of mitogen-activated protein kinase pathway activation in T cells without altering fundamental system outputs." in: Molecular and cellular biology, Vol. 29, Issue 8, pp. 2082-91, (2009) (PubMed).

Yoshida, Sakamoto, Yamashita, Arguni, Horigome, Arima, Hatano, Seki, Ichikawa, Tokuhisa: "Bcl6 controls granzyme B expression in effector CD8+ T cells." in: European journal of immunology, Vol. 36, Issue 12, pp. 3146-56, (2006) (PubMed).

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, (1997) (PubMed).

Nishio, Spielman, Lee, Nelson, Podack: "CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4347-53, (1996) (PubMed).

Nandi, Gross, Allison: "CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3361-9, (1994) (PubMed).

Ozato, Hansen, Sachs: "Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 125, Issue 6, pp. 2473-7, (1981) (PubMed).

Target Details for CD28

Target: CD28

Alternative Name: CD28 (CD28 Products)

Background: CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. (Ref.1-4) CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses. (Ref.5-8)Synonyms: T-cell-specific surface glycoprotein CD28, TP44

UniProt: P31041

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response