Vimentin antibody
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- Target See all Vimentin (VIM) Antibodies
- Vimentin (VIM)
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Reactivity
- Human, Mouse, Rat, Pig, Cow, Chicken, Monkey, Dog, Cat, Rabbit, Hamster, Horse, Gerbil
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Vimentin antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Recognizes porcine Vimentin. Predicted species cross-reactivity: Human, monkey, bovine, equine, rabbit, canine, feline, rat, mouse, hamster, gerbil and chicken.
- Purification
- Protein G purified
- Immunogen
- Vimentin from porcine eye lens. Cellular Localization: Cytoplasmic.
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product VIM Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (10 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: Sarcomas. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Pig
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4. No preservatives, no stabilizing proteins added.
- Preservative
- Azide free
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- Vimentin (VIM)
- Alternative Name
- Vimentin (VIM Products)
- Background
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Name/Gene ID: VIM
Synonyms: VIM, Vimentin - Gene ID
- 7431
- UniProt
- P08670
- Pathways
- Caspase Cascade in Apoptosis
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