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GFP antibody (HRP)

GFP Reactivity: Aequorea victoria WB, ELISA, IHC Host: Mouse Monoclonal 1003 HRP

Catalog No. ABIN364147

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
174

15

11

9
Aequorea victoria
Host
63

63

34

13

9

5

2

1
Mouse
Compatible Secondaries
Clonality
113

72
Monoclonal
Conjugate
82

12

11

9

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to HRP
Application
147

79

47

33

27

25

23

21

14

13

13

12

12

5

4

4

4

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity

Recognizes wild type and recombinant Green Fluorescent Protein. Does not recognize enhanced forms of GFP. Expected to detect GFP containing inserts by Immunofluorescence Microscopy on prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, anti-peroxidase, and purified and partially purified GFP (Aequorea victoria) serum.

Purification

Protein A purified

Immunogen

GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Type of Immunogen: Fusion protein

Clone

1003

Isotype

IgG1 kappa

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Application Notes

Approved: ELISA (1:10000 - 1:50000), IHC (1:500 - 1:2500), WB (1:1000 - 1:5000)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA (sandwich or capture): 1:10000-1:50000. Western Blot: 1:1000-1:5000. Immunohistochemistry: 1:500-1:2500. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Aequorea victoria

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Thimerosal, 40 % glycerol.

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Protect from light.

Storage

4 °C,-80 °C

Storage Comment

Short term: 4°C
Long term: -70°C
Protect from light.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Background

Name/Gene ID: GFP

Synonyms: GFP

UniProt

P42212