VEGFC antibody

Catalog No. ABIN360866

Product Details anti-VEGFC Antibody

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This VEGFC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody detects VEGF-C in Western Blots. No cross-reactivity is shown with VEGF-A.

Purification: Protein G Affinity Chromatography

Immunogen: Recombinant Human VEGF-C protein

Clone: 9-00E-007

Isotype: IgG2a

Application Details

Application Notes: Western Blot. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in Sterile Water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: Phosphate-Buffered Saline (PBS)

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to and following reconstitution store the antibody at -20 °C.

Target Details for VEGFC

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Alternative Name: VEGF-C / Flt4-L (VEGFC Products)

Background: VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The human VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant human VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant human VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant human VEGF-C contains 115 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.Synonyms: Flt4 ligand, VEGFC, VRP, Vascular endothelial growth factor C, Vascular endothelial growth factor-related protein

Gene ID: 7424

NCBI Accession: NP_005420

UniProt: P49767

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2