alpha Actinin 4 antibody (N-Term)

Catalog No. ABIN360099

Product Details anti-alpha Actinin 4 Antibody

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Actinin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to ACTN4.

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ACTN4.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Alternative Name: alpha-Actinin-4 / ACTN4 (ACTN4 Products)

Synonyms: fsgs antibody, fsgs1 antibody, C77391 antibody, ACTININ-4 antibody, FSGS antibody, FSGS1 antibody, wu:fb53f05 antibody, zgc:63508 antibody, actinin alpha 4 antibody, actinin, alpha 4 antibody, actinin alpha 4 S homeolog antibody, actn4 antibody, ACTN4 antibody, Actn4 antibody, actn4.S antibody

Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This protein is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and is thought to be involved in metastatic processes. Mutations in the gene encoding this protein have been associated with focal and segmental glomerulosclerosis.Synonyms: F-actin cross-linking protein, Non-muscle alpha actinin 4

Gene ID: 81, 9606

UniProt: O43707

Pathways: Proton Transport