Histone 3 antibody (pSer10)
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- Target See all Histone 3 (H3) Antibodies
- Histone 3 (H3)
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Binding Specificity
- pSer10
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Histone 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Purification
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Immunogen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human H3.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product H3 Primary Antibody
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anti-Histone 3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27ac) antibodyH3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (3meLys4) antibodyH3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated
anti-Histone 3 (H3) (H3K9ac) antibodyH3 Reactivity: Human, Mouse WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (AA 71-136) antibodyH3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27me) antibodyH3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (C-Term) antibodyH3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 Reactivity: Human WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
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- Application Notes
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- Histone 3 (H3)
- Alternative Name
- Histone H3 (H3 Products)
- Background
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.Synonyms: H3 Histone family, HIST1H3
- Molecular Weight
- 15404 Da
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