PCNA antibody (Middle Region)
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- Target See all PCNA Antibodies
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
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Binding Specificity
- Middle Region
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PCNA antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- This antibody detects PCNA (Center).
- Purification
- Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Immunogen
- Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product PCNA Primary Antibody
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anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 151-261) antibody
PCNA Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 231-261), (C-Term) antibodyPCNA Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB17486 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 53-196) antibodyPCNA Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 7H4F8 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) antibodyPCNA Reactivity: Human WB, IHC, IF, IHC (p), IP, ICC, IHC (fro), FISH Host: Rabbit Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibodyPCNA Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 1G7 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibodyPCNA Reactivity: Human WB, ELISA Host: Mouse Monoclonal S1 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibodyPCNA Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 201-261) antibodyPCNA Reactivity: Human, Mouse, Rat, Chicken WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 6-261) antibodyPCNA Reactivity: Human, Mouse, Rat, Pig, Rabbit, Dog, Rhesus Monkey WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) antibodyPCNA Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 1H10 unconjugated
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- Application Notes
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ELISA: 1/1,000. Western Blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
- Alternative Name
- PCNA (PCNA Products)
- Background
- PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.Synonyms: Cyclin, Proliferating Cell Nuclear Antigen
- Molecular Weight
- 28769 Da
- Gene ID
- 5111, 9606
- UniProt
- P12004
- Pathways
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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