RFC5 antibody (Middle Region)

Catalog No. ABIN357979

Product Details anti-RFC5 Antibody

Target: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFC5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects RFC5 (Center).

Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human RFC5.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for RFC5

Target: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Alternative Name: RFC5 / RFC36 (RFC5 Products)

Background: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.Synonyms: Activator 1 36 kDa subunit, Activator 1 subunit 5, RF-C 36 kDa subunit, Replication factor C 36 kDa subunit, Replication factor C subunit 5

Molecular Weight: 38497 Da

Gene ID: 5985, 9606

UniProt: P40937

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA