Vimentin antibody
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- Target See all Vimentin (VIM) Antibodies
- Vimentin (VIM)
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Vimentin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody detects Vimentin (VIM). Predicted to cross react with Mouse (100 % Antigen Homology).
- Purification
- Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Immunogen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S82 of human VIM.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product VIM Primary Antibody
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- Application Notes
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ELISA: 1/1,000. Western Blot: 1/50-1/100Immunohistochemistry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- Vimentin (VIM)
- Alternative Name
- Vimentin (VIM Products)
- Background
- Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Synonyms: VIM
- Molecular Weight
- 53652 Da
- Gene ID
- 7431, 9606
- UniProt
- P08670
- Pathways
- Caspase Cascade in Apoptosis
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