Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-term of human SULT4A1 (region common to both isoforms a and b).
SULT4A1
Reactivity: Human, Rat
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 594
Application Notes
ELISA: 1/1,000. Western Blot: 1/100-1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target
SULT4A1
(Sulfotransferase Family 4A, Member 1 (SULT4A1))
Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure(number and length of exons) is similar among family members. SULT4A1 isoforms a and b are selectively expressed in brain tissue. Isoform a is the predominant of the two isoforms of this protein. Isoform b includes an alternate segment, compared to isoform a, that causes a frameshift. The resulting protein (isoform b) has a distinct and shorter C-terminus when compared to isoform a.Synonyms: Brain sulfotransferase-like protein, NST, Nervous system sulfotransferase, ST4A1, SULTX3, Sulfotransferase 4A1