UBE2L3 antibody (N-Term)
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- Target See all UBE2L3 Antibodies
- UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This UBE2L3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody is specific to UBE2L3/UBCH7 (N-term). Predicted to cross react with Mouse (100 % Antigen Homology).
- Purification
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Immunogen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UBE2L3.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product UBE2L3 Primary Antibody
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- Application Notes
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))
- Alternative Name
- UBE2L3 / UBCH7 (UBE2L3 Products)
- Background
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.Synonyms: E2-F1, L-UBC, UBCE7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2 L3, Ubiquitin-protein ligase L3
- Molecular Weight
- 17861 Da
- Gene ID
- 7332, 5874
- UniProt
- P68036
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