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Vimentin antibody

VIM Reactivity: Human IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN356449

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Reactivity
305

120

117

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Human
Host
158

130

11

6

6

5

2
Rabbit
Compatible Secondaries
Clonality
172

145
Polyclonal
Conjugate
198

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
263

115

104

97

88

64

48

40

39

33

23

20

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

This antibody reacts with Human Vimentin. Other species not tested.
Cellular Localization: Cytoplasmic.

Purification

Protein A Chromatography.

Immunogen

Purified human Vimentin.

Isotype

IgG

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VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS, StM Host: Mouse Monoclonal VM452 unconjugated

Application Notes

Immunohistochemistry: 1/50-1/100 using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears do not requireantigen retrieval. Positive control: Sarcomas, melanomas, Hela cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

3.0 mg/mL

Buffer

10 mM PO4, 200 mM NaCl, pH 7.4, 0.05 % Sodium Azide, 1 % BSA

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target

Vimentin (VIM)

Alternative Name

Vimentin (VIM Products)

Background

Vimentin is 57 kDa intermediate filament proteins in mesenchymal cells. It is attached to the nucleus, endoplasmic reticulum and mitochondria either laterally or terminally. Vimentin is important in the differential diagnosis of undifferentiated neoplasm.Synonyms: VIM

Gene ID

7431

NCBI Accession

NP_003371

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis