Collagen Type I antibody (C-Term)

Catalog No. ABIN344730

Product Details anti-Collagen Type I Antibody

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Binding Specificity: C-Term

Reactivity: Human, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Specificity: Recognizes Collagen Type I near the C-terminal. Displays a high affinity for human, bovine and sheep Type I Collagens. There is no evidence for cross-reactivity with Collagen Types III, V and VI or connective tissue proteins (Elastin, Fibronectin and Laminin) at suggested working concentrations. Species cross-reactivity: Human bovine, sheep, porcine and canine. Does not react with mouse, rat, guinea pig, squirrel, equine, kangaroo or chicken.

Purification: Protein A purified

Immunogen: Human collagen.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1:50 - 1:250), Flo (1:100), IHC, IHC-Fr, WB

Usage: Suitable for use in ELISA, Immunohistochemistry, Flow Cytometry, and Western Blot. ELISA (Capture): 2-4 μg/mL. ELISA (Detection): 10 μg/mL will detect 50 ng of human Collagen Type I with no reactivity with other Collagens. Higher levels of antibody may be necessary to detect lower concentrations of Collagen. Immunohistochemistry (Unfixed frozen sections): 1:50-1:250. Flow Cytometry: 1:100. Western Blot (non-denatured and non-reduced): 1:500. Positive control: Human kidney lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM Tris acetate, 150 mM sodium chloride, 0.1 % Bronidox,  pH 4.5.

Preservative: Bronidox

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen I (COL1 Products)