CD14 antibody (AA 147-152)

Catalog No. ABIN343170

Product Details anti-CD14 Antibody

Target: CD14

Binding Specificity: AA 147-152

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Agonist (Agon)

Specificity: Soluble and membrane bound human CD14. The antibody does'nt significant block LPS binding to membrane bound CD14.

Purification: Protein G affinity column

Immunogen: human Monocytes

Clone: BiG2

Isotype: IgG1

Application Details

Application Notes: no significant inhibition of LPS binding to CD14

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with bidistilled water

Concentration: 1 mg/mL

Buffer: PBS, pH 7.7

Preservative: Without preservative

Storage: -20 °C,-80 °C

References for anti-CD14 antibody (ABIN343170)

Stelter, Loppnow, Menzel, Grunwald, Bernheiden, Jack, Ulmer, Schuett: "Differential impact of substitution of amino acids 9-13 and 91-101 of human CD14 on soluble CD14-dependent activation of cells by lipopolysaccharide." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 11, pp. 6035-44, (1999) (PubMed).

Dziarski, Tapping, Tobias: "Binding of bacterial peptidoglycan to CD14." in: The Journal of biological chemistry, Vol. 273, Issue 15, pp. 8680-90, (1998) (PubMed).

Neumeister, Faigle, Sommer, Zaehringer, Stelter, Menzel, Schuett, Northoff: "Low endotoxic potential of Legionella pneumophila lipopolysaccharide due to failure of interaction with the monocyte lipopolysaccharide receptor CD14." in: Infection and immunity, Vol. 66, Issue 9, pp. 4151-7, (1998) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Synonyms: CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background: Background: biG 2 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Molecular Weight: ~50kDa

Gene ID: 929

NCBI Accession: NP_000582

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades