Ubiquitin antibody

Catalog No. ABIN340405

Product Details anti-Ubiquitin Antibody

Target: Ubiquitin

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes ubiquitin, both conjugated and unconjugated. Reactivity has been shown in human, bovine, chicken, Drosophila and C. elegans.

Purification: Purified

Immunogen: Purified bovine ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin (KLH).

Type of Immunogen: Hapten

Isotype: IgG1

Application Details

Application Notes: Approved: IF (1:10), IHC, IHC-Fr, IHC-P, IP, WB (1:10)

Usage: Can be used for immunoassays, Western Blot, Immunofluorescence and Immunoprecipitation. Furthermore the antibody is useful for Immunohistology after heat-induced retrieval on both frozen and paraffin embedded sections. Immunohistology: 1:10. Immunofluorescence: 1:10. Western Blot: 1:10. For Western Blot it is recommended to use lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl, 20 mM Tris-HCl, 5 mM beta-mercaptoethanol, 1 mM PMSF (  pH 7.5)) for 30-60 minutes at 4?C, followed by extensive PBS washing.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, 0.1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Ubiquitin

Target: Ubiquitin

Alternative Name: Ubiquitin (Ubiquitin Products)

Synonyms: CG11624 antibody, DmUb antibody, DmUbi-p63E antibody, Dmel\\CG11624 antibody, Ub antibody, Ubi-P63e antibody, Ubi-p antibody, Ubi63E antibody, Ubi63p antibody, Ubip antibody, Ubiq antibody, Ubp antibody, ubi antibody, ubi-p63E antibody, ubi63E antibody, CG18282 antibody, CG32744 antibody, CR32744 antibody, DmUbi-p5E antibody, Dmel\\CG32744 antibody, ubiquitin antibody, si:dz180g5.5 antibody, si:busm1-180g5.5 antibody, LOC100194618 antibody, UBIQ antibody, Ubiquitin antibody, LOC100282068 antibody, Ubiquitin-63E antibody, Ubiquitin-5E antibody, transmembrane and ubiquitin-like domain containing 2 antibody, polyubiquitin antibody, Putative ubiquitin family protein antibody, ubiquitin antibody, ubiquitin B antibody, UBiQuitin antibody, Ubiquitin antibody, Ubi-p63E antibody, Ubi-p5E antibody, tmub2 antibody, LOC100194618 antibody, pco110026 antibody, LOC100135798 antibody, UBB antibody, ubq-1 antibody, LOC100190766 antibody

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway