CD34 antibody

Catalog No. ABIN337152

Product Details anti-CD34 Antibody

Target: CD34

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Brand: IHC-plus™

Specificity: Recognizes the mouse CD34 cell surface glycoprotein. mouse CD34 is expressed by hematopoietic progenitor cells in the bone marrow and also by endothelial cells.

Purification: Protein G purified

Immunogen: Mouse CD34

Clone: MEC14-7

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:25 - 1:50), IHC, IHC-P (10 μg/mL), IP (2 - 10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD34

Target: CD34

Alternative Name: CD34 (CD34 Products)

Background: Name/Gene ID: CD34

Synonyms: CD34, CD34 Molecule, CD34 antigen

Gene ID: 947

UniProt: P28906