ERCC1 antibody (N-Term)

Catalog No. ABIN334392

Product Details anti-ERCC1 Antibody

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ERCC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: ERCC1

Sequence: DPGKDKEGVP QPS

Specificity: This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence DPGKDKEGVPQPS-C, from the N Terminus of the protein sequence according to NP_973730.1, NP_001974.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 38 kDa band observed in lysates of cell lines A431 and Kelly (calculated MW of 35.6 kDa according to NP_973730.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: ERCC1 (ERCC1 Products)

Background: ERCC1, excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence), COFS4, UV20, excision repair cross-complementing 1, excision repair protein

Gene ID: 2067

NCBI Accession: NP_973730, NP_001974

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response