CD14 antibody (PE)

Catalog No. ABIN323076

Product Details anti-CD14 Antibody (PE)

Target: CD14

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Specificity: Recognizes porcine CD14. Mature porcine CD14 shares 70-75 % aa identity with human, cow, sheep, water buffalo and horse CD14 and 61-62 % aa identity with mouse and rat CD14.

Purification: Protein G purified

Immunogen: E. coli-derived recombinant porcine CD14.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Flow Cytometry and Direct ELISA. Western Blot: 1-2 μg/mL. The detection limit is ~25 ng/lane under non-reducing and reducing conditions. Flow Cytometry: 25 μg/mL. Add 10 μL to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. Direct ELISA: 0.5-1 μg/mL. The detection limit is ~1 ng/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Storage: -20 °C

Storage Comment: Lyophilized and resuspended forms: store at -20°C.

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Background: Name/Gene ID: CD14

Synonyms: CD14, CD14 Molecule, CD14 antigen

Gene ID: 929

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades