Tissue factor antibody (FITC)

Catalog No. ABIN320295

Product Details anti-Tissue factor Antibody (FITC)

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tissue factor antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises CD142. The CLB/TF-5 antibody has been used successfully in an ELISA assay as a coating antibody in association with clone CLB/TF-1 (Cat.-No AM01050PU-N), which recognises a different epitope of CD142.

Purification: Affinity Chromatography on Protein A from tissue culture supernantant.

Clone: CLB-TF-5

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Tris buffered saline, pH 8.0 containing, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Tissue factor antibody (FITC) (ABIN320295)

Rancourt, Veress, Guo, Jones, Hendry-Hofer, White: "Airway tissue factor-dependent coagulation activity in response to sulfur mustard analog 2-chloroethyl ethyl sulfide." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 302, Issue 1, pp. L82-92, (2012) (PubMed).

Target Details for Tissue factor

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Alternative Name: CD142 / Tissue Factor (F3 Products)

Background: CD142 is a 45kD cell surface glycoprotein which is otherwise known as Tissue Factor (TF). CD142 expression can be induced on monocytes, macrophages and endothelial cells by various stimuli including interleukin 1, tumour necrosis factor and endotoxin. CD142 initiates the blood clotting cascade by binding coagulation factor VIIa, which activates factor IX or factor X by specific limited proteolysis. CD142 also plays an important role in inflammation, angiogenesis, and the pathophysiology of atherosclerosis and cancer.Synonyms: Coagulation factor III, F3, TF, Thromboplastin

Gene ID: 2152

NCBI Accession: NP_001984

UniProt: P13726

Pathways: Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling