CD28 antibody (PE)

Catalog No. ABIN320280

Product Details anti-CD28 Antibody (PE)

Target: CD28

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig. Spleen cells from immunised CD28-/-knockout mice were fused with cells of the X63Ag8.653 myeloma cell line.

Clone: E18

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water.

Concentration: 0.1 mg/mL

Buffer: Phosphate buffered saline pH 7.4 without preservatives.

Preservative: Without preservative

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for CD28

Target: CD28

Alternative Name: CD28 (CD28 Products)

Background: Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95 % of CD4+ cells and 50 % of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 Molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

Gene ID: 10090

UniProt: P31041

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response