CD45 antibody
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- Target See all CD45 (PTPRC) Antibodies
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD45 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP)
- Specificity
- This antibody is the equivalent of human CD45. SM2195P recognises a monomorphic determinant present on all forms of the L-CA (CD45) antigen. (1) Studies have demonstrated that this antibody binds to the membrane distal two domains of CD45.(3)
- Purification
- Affinity Chromatography on Protein G.
- Immunogen
- L-CA (CD45). Fusion of cells from NSO mouse myeloma line with spleen cells from BALB/c mice immunised with purified LCA.
- Clone
- OX-29
- Isotype
- IgG2a
- Top Product
- Discover our top product PTPRC Primary Antibody
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- Application Notes
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Flow Cytometry (1/10-1/50): Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS containing 0.09 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- Alternative Name
- CD45 / LCA (PTPRC Products)
- Synonyms
- PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody
- Background
- CD45 is a major membrane glycoprotein of 180kD on thymocytes and 180-240kD on other leucocytes.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
- Gene ID
- 24699
- NCBI Accession
- NP_001103357
- UniProt
- P04157
- Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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